ParkinsonCanadaV1, Main, Exploration, bibRecord, 000B49

Amyloids of Alpha-Synuclein Affect the Structure and Dynamics of Supported Lipid Bilayers

Identifieur interne : 000B49 ( Main/Exploration ); précédent : 000B48; suivant : 000B50

Amyloids of Alpha-Synuclein Affect the Structure and Dynamics of Supported Lipid Bilayers

Auteurs : Aditya Iyer [Pays-Bas] ; Nils O. Petersen [Pays-Bas, Canada] ; Mireille M. A. E. Claessens [Pays-Bas] ; Vinod Subramaniam [Pays-Bas]

Source :

Biophysical Journal [ 0006-3495 ] ; 2014.

RBID : PMC:4070068

Abstract

Interactions of monomeric alpha-synuclein (αS) with lipid membranes have been suggested to play an important role in initiating aggregation of αS. We have systematically analyzed the distribution and self-assembly of monomeric αS on supported lipid bilayers. We observe that at protein/lipid ratios higher than 1:10, αS forms micrometer-sized clusters, leading to observable membrane defects and decrease in lateral diffusion of both lipids and proteins. An αS deletion mutant lacking amino-acid residues 71–82 binds to membranes, but does not observably affect membrane integrity. Although this deletion mutant cannot form amyloid, significant amyloid formation is observed in the wild-type αS clusters. These results suggest that the process of amyloid formation, rather than binding of αS on membranes, is crucial in compromising membrane integrity.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070068

DOI: 10.1016/j.bpj.2014.05.001
PubMed: 24940776
PubMed Central: 4070068

Affiliations:

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S. We have systematically analyzed the distribution and self-assembly of monomeric <italic>α</italic>
S on supported lipid bilayers. We observe that at protein/lipid ratios higher than 1:10, <italic>α</italic>
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S on membranes, is crucial in compromising membrane integrity.</p>
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La maladie de Parkinson au Canada (serveur d'exploration)

Amyloids of Alpha-Synuclein Affect the Structure and Dynamics of Supported Lipid Bilayers

Amyloids of Alpha-Synuclein Affect the Structure and Dynamics of Supported Lipid Bilayers

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